Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2562167 | Pharmacological Research | 2011 | 12 Pages |
Abstract
Some antibiotics, including minocycline, have recently been reported to display immunomodulatory properties in addition to their antimicrobial activity. The use of a compound with both immunomodulatory and antibacterial properties could be very interesting in the treatment of inflammatory bowel disease (IBD), so the aim of our study was to evaluate the anti-inflammatory effect of minocycline in several experimental models of IBD. Firstly, the immunomodulatory activity of the antibiotic was tested in vitro using Caco-2 intestinal epithelial cells and RAW 264.7 macrophages; minocycline was able to inhibit IL-8 and nitrite production, respectively. In vivo studies were performed in trinitrobenzenesulfonic acid (TNBS)-induced rat colitis and dextran sodium sulfate (DSS)-induced mouse colitis. The results revealed that minocycline exerted an intestinal anti-inflammatory effect when administered as a curative treatment in the TNBS model, modulating both immune and microbiological parameters, being confirmed in the DSS model; whereas none of the other antibiotics tested (tetracycline and metronidazole) showed anti-inflammatory effect. However, minocycline administration before the colitis induction was not able to prevent the development of the intestinal inflammation, thus showing that only its antimicrobial activity is not enough for the anti-inflammatory effect. In conclusion, minocycline displays an anti-inflammatory effect on different models of rodent colitis which could be attributed to the association of its antibacterial and immunomodulatory properties.
Keywords
TNFαLPSDSSIL-LipopolyssacharideCOX-2TNBSICAM-1IBDDAIiNOSMCP-1MPORAW 264.7 cellsTrinitrobenzenesulfonic acidinterleukinInflammatory bowel diseaseTetracyclinetumor necrosis factor αCaco-2 cellsinducible nitric oxide synthasedextran sodium sulfateCyclooxygenase-2disease activity indexMetronidazoleintercellular adhesion molecule-1myeloperoxidaseMinocyclinecytokine-induced neutrophil chemoattractant-1Monocyte chemotactic protein-1CINC-1Experimental colitis
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Authors
Natividad Garrido-Mesa, Desirée Camuesco, Belén Arribas, Mònica Comalada, Elvira Bailón, Margarita Cueto-Sola, Pilar Utrilla, Ana Nieto, Antonio Zarzuelo, MarÃa Elena RodrÃguez-Cabezas, Julio Gálvez,