| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 25628 | Journal of Biotechnology | 2006 | 5 Pages |
Abstract
We report a protocol for pre-fractionation of proteins from frozen tumours. Using this protocol two separate protein fractions are extracted: Fraction 1 is enriched for cytosolic and Fraction 2 for nuclear/nuclear membrane proteins. The accuracy and reproducibility of the protocol were demonstrated by Western blot analyses and SELDI–TOF-MS profilings. The resulting protein profiles were similar within the respective groups of Fractions 1 and 2 samples, without differences between freshly prepared tumours or those obtained as frozen tissue from biobanks.
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Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Lars Forsberg, Catharina Larsson, Anastasios Sofiadis, Rolf Lewensohn, Anders Höög, Janne Lehtiö,