Article ID Journal Published Year Pages File Type
2562914 Pharmacological Research 2008 5 Pages PDF
Abstract

Potassium channel openers, e.g. cromakalim are held to relax smooth muscle by hyperpolarizing the cell membrane via activation of ATP-sensitive K+ (KATP) channels. A recent report indicates that members of this group dilate cerebral arteries also by enhancing the KCa-based spontaneous transient outward currents (STOCs) through the activation of mitochondrial KATP channels. We extended the study to rat saphenous arterial myocytes, a model for peripheral resistance vessels, to investigate the effects of cromakalim on KATP and STOCs, and the underlaying mechanisms. Smooth muscle myocytes were enzymatically dissociated from the saphenous branch of the femoral artery. Macroscopic currents were recorded from acutely isolated cells using the perforated-patch and whole-cell variants of the patch-clamp technique. Predictably metabolic inhibitors and cromakalim activated a background K+ current blocked by glibenclamide, identified as the KATP channel. However, in addition, cromakalim markedly increased the amplitude and frequency of STOCs. The latter action was not sensitive to the specific KATP channel blocker glibenclamide, excluding the participation of mitocondrial KATP channels in this action. In conclusion, this study suggests that, in addition to the opening of KATP channels, the increased STOC activity may have an important role in the vasorelaxing action of cromakalim, but through a mechanism different from that reported on cerebral artery.

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