Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25641 | Journal of Biotechnology | 2006 | 9 Pages |
Abstract
We have cloned the gene that encodes a novel glucosyl transferase (AraGT) involved in rhamnosylation of the polyketide antibiotic Aranciamycin in Streptomyces echinatus. AraGT comprises two domains characteristic of bacterial glycosyltranferases. AraGT was synthesized in E. coli as a decahistidinyl-tagged polypeptide. Purified AraGT is dimeric, displays a Tmapp of 30 °C and can glycosylate the aglycone of an Aranciamycin derivative as shown by liquid chromatography and mass spectrometry. The availability of functional AraGT will allow the generation Aranciamycin-based combinatorial libraries.
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Authors
Giorgos Sianidis, Sven-Eric Wohlert, Charalambos Pozidis, Spyridoula Karamanou, Andriy Luzhetskyy, Andreas Vente, Anastassios Economou,