Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25744 | Journal of Biotechnology | 2006 | 4 Pages |
Abstract
A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Federico Katzen, Wieslaw Kudlicki,