Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
25764 | Journal of Biotechnology | 2006 | 10 Pages |
Glucose oxidase (GOD) was immobilized on cellulose acetate–polymethylmethacrylate (CA–PMMA) membrane. The immobilized GOD showed better performance as compared to the free enzyme in terms of thermal stability retaining 46% of the original activity at 70 °C where the original activity corresponded to that obtained at 20 °C. FT-IR and SEM were employed to study the membrane morphology and structure after treatment at 70 °C. The pH profile of the immobilized and the free enzyme was found to be similar. A 2.4-fold increase in Km value was observed after immobilization whereas Vmax value was lower for the immobilized GOD. Immobilized glucose oxidase showed improved operational stability by maintaining 33% of the initial activity after 35 cycles of repeated use and was found to retain 94% of activity after 1 month storage period. Improved resistance against urea denaturation was achieved and the immobilized glucose oxidase retained 50% of the activity without urea in the presence of 5 M urea whereas free enzyme retained only 8% activity.