Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
2598573 | Toxicology Letters | 2015 | 9 Pages |
Abstract
Hypoxia/reoxygenation (H/R) injury of cholangiocytes causes serious biliary complications during hepatobiliary surgeries. Molecular hydrogen (H2) has been shown to be effective in protecting various cells and organs against oxidative stress injury. Human liver cholangiocytes were used to determine the potential protective effects of hydrogen against cholangiocyte H/R injury and explore the underlying mechanisms. We found that H2 ameliorated H/R-induced cholangiocytes apoptosis. Our study revealed that H2 activated NF-E2-related factor 2 (Nrf2) and downstream cytoprotective protein expression. However, the protective function of H2 was abolished when Nrf2 was silenced. Apoptosis in cholangiocytes isolated from a rat model of liver ischemia/reperfusion injury indicated that H2 significantly attenuates ischemia/reperfusion cholangiocyte injury in vivo. In conclusion, our study shows that H2 protects intrahepatic cholangiocytes from hypoxia/reoxygenation-induced apoptosis in vitro or in vivo, and this phenomenon may depend on activating Nrf2 expression.
Keywords
CK7glutathione S-transferase alpha 2Prdx1MDAGsta2G6PCCK19NQO1AQP1EpCAMBcl2ICAM1MnSODHRSHRMNrf2HO-1ALBB-cell CLL/lymphoma 2H/RI/RROSAlbuminaquaporin 1ischemia/reperfusionBaxOxidative stressnormal salineSODManganous superoxide dismutaseSuperoxide dismutaseNF-E2-related factor 2malondialdehydeIntercellular adhesion molecule 1epithelial cell adhesion moleculeheme oxygenase 1hydrogen-rich salineMolecular hydrogenhypoxia/reoxygenationPeroxiredoxin 1BCL2-associated X proteinkeratin 19glucose-6-phosphataseReactive oxygen species
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Authors
Jianhua Yu, Weiguang Zhang, Rongguo Zhang, Guixing Jiang, Haijun Tang, Xinxian Ruan, Peitu Ren, Baochun Lu,