| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 29944 | Journal of Photochemistry and Photobiology B: Biology | 2014 | 12 Pages |
•Mutation changes singlet BLUF domain photo-cycle to triplet based photo-dynamics.•Three conformations of SUMO-bPAC-Y7F with different photo-dynamics are observed.•First fraction follows triplet based flavin semiquinone photo-cycle dynamics.•Second fraction forms irreversible flavin hydroquinone charge transfer complex.•Third fraction undergoes blue-light induced reversible flavin-C4a adduct formation.
The photoactivated cyclase bPAC of the microbial mats bacterium Beggiatoa sp. consists of a BLUF domain and an adenylyl cyclase domain. It has strong activity of photo-induced cyclic adenylyl monophosphate (cAMP) formation and is therefore an important optogenetic tool in neuroscience applications. The SUMO-bPAC-Y7F mutant where Tyr-7 is replaced by Phe-7 in the BLUF domain has lost the typical BLUF domain photo-cycle dynamics. Instead, the investigated SUMO-bPAC-Y7F mutant consisted of three protein conformations with different triplet based photo-dynamics: (i) reversible flavin quinone (Fl) cofactor reduction to flavin semiquinone (FlH), (ii) reversible violet/near ultraviolet absorbing flavin photoproduct (FlA) formation, and (iii) irreversible red absorbing flavin photoproduct (FlC) formation. Absorption and emission spectroscopic measurements on SUMO-bPAC-Y7F were carried out before, during and after light exposure. Flavin photo-dynamics schemes are developed for the SUMO-bPAC-Y7F fractions performing photo-induced FlH, FlA, and FlC formation. Quantitative parameters of the flavin cofactor excitation, relaxation and recovery dynamics in SUMO-bPAC-Y7F are determined.
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