Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
30462 | Journal of Photochemistry and Photobiology B: Biology | 2013 | 7 Pages |
•This study was performed to reposition drugs approved for other indications as anticancer agents.•The 5-ASA molecule binds with the minor groove of the duplex CT-DNA (GC region).•Several spectroscopic techniques have been used in this research.
The interaction of CT-DNA with the drug mesalamine (5-ASA) at physiological pH has been investigated by absorption, emission, circular dichroism (CD), cyclic voltammetry (CV), viscosity studies and molecular modeling. Thermodynamic parameters (ΔH > 0 and ΔS < 0) indicated that hydrogen bond and van der Waals play main roles in the binding of 5-ASA to CT-DNA. Ethidium bromide (EB) displacement studies revealed that 5-ASA did not have any effect on ethidium bromide (EB) bound DNA which is indicative of groove binding. The results obtained from experimental and molecular modeling showed that 5-ASA is a minor groove binder of DNA and preferentially binds to GC rich regions.
Graphical abstractThe interaction of CT-DNA with the drug mesalamine has been investigated. The results showed that this drug is a minor groove binder of DNA and preferentially binds to GC rich regions.Figure optionsDownload full-size imageDownload as PowerPoint slide