Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
30625 | Journal of Photochemistry and Photobiology B: Biology | 2013 | 7 Pages |
IntroductionThe effects of LLLT were studied during muscle regeneration through gene expression.MethodsIt was evaluated 10 and 50 J/cm2 doses during 7, 14 and 21 days post cryoinjury, through histopathological analysis and mRNA MyoD, Myogenin, Vegf and Cox-2 expression.ResultsIrradiated groups presented less inflammatory process than control group after 14 and 21 days. Cox-2 levels were downregulated in all irradiated groups after 7, 14 and 21 days. On day 7, both treated groups had a downregulation of Vegf levels, and an upregulation after 14 and 21 days, mainly with 50 J/cm2. The MyoD levels were upregulated with high dose in all periods and with low dose after 21 days. Myogenin expression was downregulated in both treated groups after 7 days, and was upregulated with 10 J/cm2 after 21 days.ConclusionThese responses suggest that LLLT can improve the skeletal muscle regeneration through the gene expression stimulation.
► We examine changes in the level of MRFs during muscle regeneration. ► Low-level laser therapy is able to decrease the mRNA Cox-2 levels. ► We report a cell response photo-induced.