Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
30626 | Journal of Photochemistry and Photobiology B: Biology | 2013 | 8 Pages |
In the search for natural inhibitors of plant growth, we investigate the mechanism of action of the natural furoquinoline alkaloids isolated from Balfourodendron riedelianum (Rutaceae): evolitrine (1), kokusaginine (2), γ-fagarine (3), skimmianine (4) and maculosidine (5) on the photosynthesis light reactions. Their effect on the electron transport chain on thylakoids was analyzed. Alkaloids 1, 2, 4 and 5 inhibited ATP synthesis, basal, phosphorylating and uncoupled electron transport acting as Hill reaction inhibitors on spinach chloroplasts. Alkaloid 3 was not active. The inhibition and interaction site of alkaloids 1, 2, 4 and 5 on the non-cyclic electron transport chain was studied by polarography and fluorescence of the chlorophyll a (Chl a). The results indicate that the target for 1 was localized on the donor and acceptor side of PS II. In addition alkaloids 2 and 5 affect the PS I electron acceptors on leaf discs.
► Furoquinoline were isolated from a Brazilian tree Balfourodendron riedelianum. ► In the search for photosynthetic inhibitors, the effect of alkaloids was investigated. ► The techniques pollarography and chlorophyll a fluorescence were used. ► The data was analyzed by the “JIP-test analysis”.