Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3065104 | Journal of Neuroimmunology | 2008 | 7 Pages |
Abstract
We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1–11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1–11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.
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Authors
Nina Movsesyan, Mikayel Mkrtichyan, Irina Petrushina, Ted M. Ross, David H. Cribbs, Michael G. Agadjanyan, Anahit Ghochikyan,