| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 30822 | Journal of Photochemistry and Photobiology B: Biology | 2006 | 14 Pages |
Abstract
Fluorescence quenching methods are useful to obtain information about the conformational and/or dynamic changes of proteins in complex macromolecular systems. In this review steady-state methods are described and the data interpretation is thoroughly discussed. As a special case of fluorescence quenching mechanism, fluorescence resonance energy transfer (FRET) phenomenon is also presented. Application of a FRET based method to characterize the temperature dependence of the flexibility of protein matrix is clearly demonstrated.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
László Mátyus, János Szöllősi, Attila Jenei,