The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B

The study investigated the protective activity of red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on multiple doses of ultraviolet radiation (UV)-B-induced deleterious effects in SKH-1 mice skin.Eighty 8-weeks-old female SKH-1 mice were divided into 8 groups: control, vehicle, UV-B irradiated, vehicle + UV-B irradiated, BM 2.5 mg polyphenols (PF)/cm2 + UV-B irradiated, BM 4 mg PF/cm2 + UV-B irradiated, UV-B + BM 2.5 mg PF/cm2, UV-B + BM 4 mg PF/cm2. The extract was applied topically before or after each UV-B exposure (240 mJ/cm2), for 10 days consecutively. The antioxidant activity of BM extract is higher than gallic acid (kBM = 0.017, kgallic acid = 0.013). Multiple doses of UV-B generated the formation of cyclobutane pyrimidine dimers (CPDs) and sunburn cells, increased glutathione peroxidase (GPx) and catalase (CAT) activities respectively glutathione (GSH) and IL-1β levels in skin. In group treated with 2.5 mg PF/cm2 before UV-B irradiation BM extract inhibited UV-B-induced sunburn cells, restored the superoxide dismutase (MnSOD) activity, increased insignificantly CAT and GPx activities and reduced IL-1β level. The BM 4.0 mg PF/cm2 treatment decreased GSH level and reduced the percentage of CPDs positive cells in skin. Both doses of BM extract administered after UV-B irradiation increased the MnSOD and GPx activities and reduced the formation of sunburn cells in skin.Our results suggest that BM extract might be a potential chemo-preventive candidate in reducing the oxidative stress and apoptosis induced by multiple doses of UV-B in skin.

► In vitro the BM extract exhibited appreciable antioxidant activity. ► Pretreatment with BM extract inhibited UVB-induced sunburn and CPDs positive cells. ► Low dose of BM extract restored the MnSOD activity and reduced the IL-1β level. ► BM extract applied after UVB irradiation increased the MnSOD and GPx activities. ► BM extract might a potential candidate with photochemopreventive effect in skin.