| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3286 | Biochemical Engineering Journal | 2013 | 8 Pages |
•A novel technology was developed for direct separation of tanshinones from TCMs.•This technology integrated extraction and cleanup into one step.•This technology improved solvent extraction of tanshinones at room temperature.•This technology had higher extraction efficiency than conventional extraction.•This technology provided reusability of solvent.
A novel separation technology at room temperature for traditional Chinese medicines was proposed in this work by adding magnetic molecularly imprinted polymers (M-MIPs) into extraction solution and sample matrix. The M-MIPs show a more adsorption capacities and higher selectivity for the template than magnetic non-molecularly imprinted polymers (M-NMIPs) without the specific binding sites. Addition of the M-MIPs to the extraction solution provides one-step extraction and cleanup, the improvement of extraction rate and extraction yields of three tanshinones (from 0.40, 0.23 and 0.12 mg g−1 in 240 min by solvent extraction to 0.52, 0.27 and 0.19 mg g−1 in 5 min by 200 mg sorbent), and reusability of extraction solvent. The extraction yields of three tanshinones by this technology at room temperature in 5 min were higher than those by ultrasonic extraction in 30 min, by heat reflux extraction in 45 min and by solvent extraction at room temperature in 4 h. The integrated technology has the advantages of one-step extraction and cleanup, high extraction efficiency, low solvent consumption and room temperature.
