Article ID Journal Published Year Pages File Type
3369592 Journal of Clinical Virology 2011 5 Pages PDF
Abstract

BackgroundAcute infectious mononucleosis (AIM) is generally associated with a large EBV B cell reservoir cells and an intense B-cell polyclonal activation whereas the number of quiescent EBV-infected memory B cells in chronically EBV-infected healthy controls is very low.ObjectivesTo evaluate the extent and functionality of ex vivo B-cell polyclonal activation, quantify the EBV DNA integrated in B cells, enumerate the functional EBV DNA reservoir in B cells and circulating B cells spontaneously secreting EBV antigens in AIM.Study designCirculating B cells and B cells differentiating into plamablasts and plasma cells, early (BZLF1)- and late viral antigen (gp350)-secreting-cells (SCs) were enumerated in six AIM patients and seven healthy EBV carriers.ResultsIn vitro B-cell polyclonal activation induced 8000–24,000 BZLF1- and 1000–3000 gp350-SCs/106 B cells, respectively. These data suggest that only 11.1–19.5% of cells expressing BZLF1 synthesized gp350 and so completed the EBV-lytic cycle. Furthermore, circulating spontaneous BZLF1- and gp350-SCs that reflect ongoing viral replication were rare (20–120 and 10–30/106 B cells, respectively), and their low numbers contrasted with the high levels of circulating plasma cells (1.1–10.2% of CD19+ B cells).ConclusionThe in vivo terminal-B-cell differentiation into plasma cells could unmask EBV B-cell reservoir to specific cytotoxic T-cell response and combined with a predominant abortive functional-EBV-reservoir, strongly contribute to rapid decay of cellular EBV reservoir in AIM.

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