| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 33702 | New Biotechnology | 2009 | 5 Pages |
Abstract
Recent years have seen tremendous progress in next generation sequencing technologies, allowing genomic sequencing in a highly cost-effective manner. However, sample preparation for these sequencers remains a bottleneck as the human genome is too complex to be routinely resequenced. We present here an in-depth study of HybSelect™, a method that can specifically enrich a large number of genes or regions of interest from any chromosomal DNA. The study used Escherichia coli K12 MG1655 as a model organism to test parameters such as method fidelity, capacity or reproducibility as a proof-of-principle.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Nadine Schracke, Tobias Kornmeyer, Marcel Kränzle, Peer F. Stähler, Daniel Summerer, Markus Beier,