Production of high level of cellulase-poor xylanases by wild strains of white-rot fungus Coprinellus disseminatus in solid-state fermentation

The production, optimisation and partial characterisation of xylanases from newly isolated wild strains of Coprinellus disseminatus was performed in solid-state fermentation. Strains SH-1 and SH-2 showed high xylanase (727.78 and 227.99 IU/mL) with very low CMCase (0.925 and 0.660 IU/mL) and laccase (0.640 and 0.742 U/mL) activities at incubation time seven days, 37°C and initial pH 6.4, using yeast extract as nitrogen source and cheap substrate (wheat bran), which increased the cost effectiveness of the process. Crude cellulase-poor xylanases obtained from test strains showed maximum activities at 55°C and pH 6.4 and retained 32.64 (SH-1) and 35.03% (SH-2) activity at pH 8 and 43.01 (SH-1) and 25.00% (SH-2) activity at 65°C. As test strains produced high level of cellulase-poor xylanases, which were active over a wide range of temperature and pH, these enzymes might be used as pulp biobleaching agents.