| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3394926 | Anaerobe | 2015 | 5 Pages |
•Clostridium difficile was isolated from 13.67% faecal samples of pet dogs in Assam.•62.5% (10/16) isolates were toxigenic and they belonged to three different ribotypes.•Toxigenic isolates carried both tcdA and tcdB and none carried binary toxin genes.•Antibiotic treatment had significant influence on isolation rate of C. difficile.•PFGE analysis revealed that each of the ribotype belonged to the same clone.
One hundred and seventeen faecal samples from pet dogs (pup = 21 and adult = 96) brought for treatment to a veterinary clinic were examined for Clostridium difficile. A total of 16 (13.67%) samples were positive. Nine (56.25%) isolates were obtained from 17 adult dogs undergoing antibiotic treatment and this was significantly higher (p < 0.01) as compared to isolates from dogs without antibiotic treatment. Ten isolates (62.5%) were toxigenic (all toxinotype 0) and six were non-toxigenic. None of the isolates were positive for binary toxin genes. PCR ribotyping revealed three different ribotypes (012, 014 and 046) among A+B+ isolates and five different ribotypes (010, SLO 131, and ACD 001 to ACD 003) among A−B− isolates. The PFGE analysis of toxigenic isolates revealed three different pulsotypes corresponding to the PCR ribotypes.
