Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3396759 | Clinical Microbiology and Infection | 2013 | 4 Pages |
Abstract
A rapid and sensitive H7 and N9 subtype-specific reverse transcription loop-mediated isothermal amplification assay was developed respectively for visual detection of human-infected influenza A (H7N9) virus. The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of hydroxynaphthol blue dye before amplification. The detection limits of both subtype-specific assays were comparable to those of validated H7 and N9 real-time PCR assays respectively and no cross-detection was observed with influenza A pandemic H1N1, H3N2, H5N1, H9N2 or influenza B virus. The assays were evaluated further with H7N9 virus-infected clinical specimens.
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Authors
K. Nie, X. Zhao, X. Ding, X.D. Li, S.M. Zou, J.F. Guo, D.Y. Wang, R.B. Gao, X.Y. Li, W.J. Huang, Y.L. Shu, X.J. Ma,