Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3398137 | Clinical Microbiology and Infection | 2009 | 4 Pages |
Abstract
The present study aimed to improve the rate of detection of blood-borne microbes by using PCRs with pan-bacterial and Candida specificity. Seventeen per cent of the blood samples (n = 178) collected from 107 febrile patients with haematological malignancies were positive using standard culture (BacT/Alert system). Candida PCR was positive in 12 patients, only one of whom scored culture-positive. Bacterial PCR using fresh blood samples was often negative, but the detection rate increased when the blood was pre-incubated for 2 days. These data indicate that PCR assays might be a complement for the detection of blood-borne opportunists in immunocompromised haematology patients.
Keywords
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Microbiology
Authors
S. Skovbjerg, C. Welinder-Olsson, N. Kondori, E. Kjellin, F. Nowrouzian, A.E. Wold, D. Stockelberg, P. Larsson, C. Wennerås,