Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3407307 | Journal of Virological Methods | 2010 | 5 Pages |
Abstract
Allele-specific PCR based on subtype consensus sequences is a powerful technique for detecting low frequency drug resistant mutants in HIV-1 infected patients. However, this approach can be limited by genetic variation in the region complementary to the primers, leading to variability in allele detection. The goals of this study were to quantify this effect and then to improve assay performance.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Valerie F. Boltz, Frank Maldarelli, Neil Martinson, Lynn Morris, James A. McIntyre, Glenda Gray, Mark J. Hopley, Toshio Kimura, Douglas L. Mayers, Patrick Robinson, John W. Mellors, John M. Coffin, Sarah E. Palmer,