| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3416353 | Microbial Pathogenesis | 2016 | 13 Pages |
•PA0011 plays a global role in antibiotics resistance and virulence in a temperature-regulated manner in PAO1.•The expression of PA0011 is higher at an environment temperature (21 °C) than that at a body temperature (37 °C).•The impaired integrity and stability of outer membrane in PA0011 mutant contributes to the reduced antibiotics resistance.•PA0011 plays a negative regulatory role in type 3 secretion system in Pseudomonas aeruginosa.
Pseudomonas aeruginosa is an important human pathogen which adapts to changing environment, such as temperature variations and entering host by regulating their gene expression. Here, we report that gene PA0011 in P. aeruginosa PAO1, which encodes a 2-OH-lauroytransferase participating in lipid A biosynthesis, is involved in carbapenem resistance and virulence in a temperature-regulated manner in PAO1. The expression of PA0011 was higher at an environment temperature (21 °C) than that at a body temperature (37 °C). The inactivation of PA0011 rendered increased antibiotic susceptibility and decreased virulence both in vivo and in vitro. The impaired integrity and the decreased stability of the outer membrane were the cause of the increased susceptibility of PAO1(Δ0011) to carbapenem and many other common antibiotics. The reduced endotoxic activity of lipopolysaccharide (LPS) contributed to the decreased virulence both at 21 °C and 37 °C in PAO1 (Δ0011). In addition, we have found that PA0011 repressed the expression of TTSS virulence factors both at transcriptional and translational levels, similar to the effect of O antigen of LPS but unlike any effect of its homologue reported in other bacteria. The effect of PA0011 on resistance to many antibiotics including carbapenem and virulence in P. aeruginosa makes it a target for novel antimicrobial therapies.
