| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 3416478 | Microbial Pathogenesis | 2015 | 11 Pages |
•A. radioresistens actively release OMVs in distinctive size populations.•OMV proteins were derived from cytosol, IM, periplasm, OM and extracellular region.•OMV proteome revealed an association of few proteins with biofilm and cytotoxicity.•OMVs act as ‘gluing material’ to increase the chances of cell to cell adhesion.•OMVs also possess a potential to damage the human epithelial kidney cells (Hek293).
Acinetobacter radioresistens is an important member of genus Acinetobacter from a clinical point of view. In the present study, we report that a clinical isolate of A. radioresistens releases outer membrane vesicles (OMVs) under in vitro growth conditions. OMVs were released in distinctive size ranges with diameters from 10 to 150 nm as measured by the dynamic light scattering (DLS) technique. Additionally, proteins associated with or present into OMVs were identified using LC-ESI-MS/MS. A total of 71 proteins derived from cytosolic, cell membrane, periplasmic space, outer membrane (OM), extracellular and undetermined locations were found in OMVs. The initial characterization of the OMV proteome revealed a correlation of some proteins to biofilm, quorum sensing, oxidative stress tolerance, and cytotoxicity functions. Thus, the OMVs of A. radioresistens are suggested to play a role in biofilm augmentation and virulence possibly by inducing apoptosis.
