Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3419188 | Revista Iberoamericana de Micología | 2007 | 4 Pages |
Abstract
We have studied 25 clinical isolates of C. albicans from oral cavities, blood, skin, nail, stool, oesophagus biopsy and vaginal fluids from patients suffering from candidiasis. For each strain biofilm formation was analysed by measuring the ability to adhere to and grow on polystyrene plastic surfaces using XTT 2,3-bis (2-methoxi-4nitro-5sulfophenil) -2H tetrazolium-5carboxanilide. reduction assay. The similarity coefficients generated by RAPD using four different primers varied from 49 to 91%, indicating a high degree of genetic variability between the clinical isolates. The dendrogram clustered the isolates in four related groups, all groups included strains with very different abilities to form biofilms. The isolates with similar genotypes often showed very different biofilm formation abilities. Strains were grouped into clusters independently of their clinical sources. Our results suggested that a direct correlation does not exist between the biofilm-forming ability of natural populations of C. albicans and the genotype as determined by RAPD.
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Authors
Estela Liliana Durán, Maria Teresa Mujica, Virginia Marta Jewtuchowicz, Jorge Luis Finquelievich, Maria Victoria Pinoni, Cristina Adela Iovannitti,