| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 34223 | Process Biochemistry | 2015 | 6 Pages |
•Cyst germination method was used for efficient astaxanthin extraction in outdoors.•Light was a limiting factor for the germination rate under autotrophic conditions.•Astaxanthin concentration was highly stable during autotrophic germination.•Astaxanthin extraction efficiency was enhanced by dividing cysts.
Haematococcus pluvialis is the richest source of natural astaxanthin (3S, 3′S), but the rigid cell wall of mature red cyst (aplanospore) complicates the efficient extraction of astaxanthin from the strain. Herein, the cyst germination method was developed by using nitrate and light for practical application for an efficient astaxanthin extraction from Haematococcus cells cultured in outdoor condition where flue gas, solar radiation and photobioreactor were used. Notably, autotrophic germination rate was easily regulated by light intensity. Under conditions of low germination rate, total astaxanthin concentration was highly maintained and astaxanthin extraction efficiency was rapidly enhanced during autotrophic germination. As a result, under homogenization (30 s), the extracted astaxanthin concentration in the cells treated with 1 mM KNO3–150 μE/m2/s was highly increased by 58% compared to the cells treated without germination. Our technical solution will definitely improve an astaxanthin extraction titer with the practical application in outdoor Haematococcus culture system.
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