| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 34361 | Process Biochemistry | 2015 | 8 Pages |
•We reveal the hypoglycemic mechanisms of GvEx in high-glucose-induced insulin-resistant liver cells.•Insulin-resistant status was improved by GvEx through modulating the insulin signaling cascade.•GvEx increases glucose uptake and enhanced glycogen accumulation.
The effects of aqueous guava leaf extract (GvEx) on insulin resistance were evaluated in high glucose-induced insulin-resistant mouse FL83B cells. Glucose uptake and glycogen content were determined and western blot analysis was performed to study the anti-diabetic effects of GvEx. Our results showed that administration of 200 and 400 μg/mL GvEx resulted in a significant increase in glucose uptake in both normal and insulin-resistant cells. Glycogen content in cells was significantly higher after incubation with GvEx (200, 400 μg/mL) than that in control group (p < 0.05). Further, western blot analysis revealed that 200 and 400 μg/mL GvEx significantly enhanced the levels of insulin-related signaling components, including insulin receptor (IR), as well as enhancing the phosphorylation of the insulin receptor (p-IR (Tyr)), insulin receptor substrate (p-IRS (Tyr)), p85 regulatory subunit of phospho-inositide 3 kinase (PI3K (p85)), protein kinase B (p-Akt (Ser)), glucose transporter 2 (Glut 2), and total glycogen synthase. In conclusion, GvEx improved insulin resistance by promoting glucose uptake and enhancing glycogen content via modulation of the insulin signaling pathway in high glucose-induced insulin-resistant mouse FL83B cells.
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