| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 34616 | Process Biochemistry | 2013 | 5 Pages |
•Residual methylene chloride in HHT was removed through pre-treatment with ethanol.•Residual methylene chloride was effectively removed.•Residual methylene chloride easily met the ICH-specified value.
In this study, methylene chloride, which is a residual solvent in final purified homoharringtonine, was removed effectively through pre-treatment with ethanol. When the final HPLC-purified sample was concentrated using a rotary evaporator, the residual methanol easily met the ICH-specified value (3000 ppm), but methylene chloride did not meet the ICH-specified value (600 ppm). However, when the sample (methylene chloride: 10,000 ppm, methanol: 500 ppm) was concentrated through pre-treatment with 95% ethanol using a rotary evaporator, the residual methylene chloride easily met the ICH-specified value. Also, the residual ethanol (concentration > 10,000 ppm) was removed effectively below the ICH-specified value (5000 ppm) through microwave-assisted drying (microwave power: 400 W).
