Article ID Journal Published Year Pages File Type
34659 Process Biochemistry 2014 6 Pages PDF
Abstract

•The PoPMuSiC algorithm was applied for rational engineering of the Bacillus catalase.•Lys114 was mutated to increase the catalytic efficiency.•The variant Lys114Tyr showed great potential in the production of industrial catalase.

Alkaline catalases with good properties are desirable in the textile industry. In the present work, by applying the PoPMuSiC algorithm for the calculation of the folding free energy, Lys114 of a Bacillus catalase was rationally selected and engineered to improve the thermostability. Interestingly, the Lys114Tyr, Lys114Val, Lys114Met and Lys114Ile variants showed higher catalytic efficiencies when compared with the wild-type protein. In particular, the Lys114Tyr variant showed the highest catalytic efficiency, which was 5.3-fold of the wild-type catalase. The production of the Lys114Tyr variant may represent an improved catalase suitable for industrial purposes.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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