Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
35067 | Process Biochemistry | 2009 | 8 Pages |
A surfactin mono-methyl ester was isolated from the cell broth of Bacillus subtilis HSO121 by acid precipitation, methanol extraction and two-step chromatographic methods. The structure was analyzed by GC/MS, HPLC, ESI Q-TOF MS/MS and NMR. It indicates that this ester is a C15-surfactin-O-methyl ester of which the fatty acid portions are composed of iso-C15 and anteiso-C15 β-hydroxy fatty acids. Based on the critical micelle concentration (CMC) in phosphate buffered saline (PBS, pH 8.0) and the IC50 value on Hela cell lines, the C15-surfactin-O-methyl ester has a stronger surface activity and lower antitumoral effect than C15-surfactin does. It is found that the Glu residues of surfactin-like lipopeptides play a role in their anti-proliferative effects on Hela cells. The possible antitumoral activity of surfactin is discussed in relation to their structures.