Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
35151 | Process Biochemistry | 2012 | 8 Pages |
To renature the inactive rhBMP-2 which overexpressed in Escherichia coli, post-expression treatments including inclusion bodies solubilization and in vitro refolding were systematically investigated. An optimized refolding process was established from screening and successfully scaled up with yield greater than 70%. Then, hydrophobic interaction chromatography (HIC) was adopted as two consecutive stages to separate the active rhBMP-2 homodimer from refolding mixture. Aiding additive N,N-dimethylformamide (DMF) was found to enhance the resolution of rhBMP-2 homodimer most effectively. The rhBMP-2 homodimer was purified to homogeneity through two HIC separations at different salt contents, the purified rhBMP-2 homodimer was fully bioactive and had equivalent biological activity to rhBMP-2 produced from Chinese hamster ovary cell (CHO). Under the optimal refolding and purification conditions, 80 mg rhBMP-2 homodimer with high purity could be obtained from 1 g wet weight of inclusion bodies. Finally, this efficient refolding and purification procedure was successfully scaled up in the pilot pharmaceutical plant.
► RhBMP-2 homodimer was refolded effectively by simple dilution with yield 70%. ► A novel two-step DMF-aided hydrophobic interaction chromatography method was adopted. ► The active rhBMP-2 homodimer was separated from refolding mixture to homogeneity. ► The purification process was easy to scale up in industrial application.