Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
35709 | Process Biochemistry | 2007 | 4 Pages |
Abstract
In this paper, immobilized lipase catalyzed biodiesel production from lard was studied. Using Candida sp. 99-125, the effect of temperature, water content, enzyme amount, solvent and three-step methanolysis were investigated. The optimal conditions for processing 1 g of lard were: 0.2 g immobilized lipase, 8 ml n-hexane as solvent, 20% water based on the fat weight, temperature 40 °C, and three-step addition of methanol. As a result, the fatty acid methyl esters (FAMEs) yield was 87.4%. The lipase was proved to be stable when used repeatedly for 180 h.
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Authors
Jike Lu, Kaili Nie, Feng Xie, Fang Wang, Tianwei Tan,