Article ID Journal Published Year Pages File Type
36445 Process Biochemistry 2005 6 Pages PDF
Abstract

Fusarium sp. BU-011, as a high-yielding strain for d-pantolactone hydrolase production was screened from 19 Fusarium sp. strains. The conditions for enzyme formation were studied. The most suitable carbon source for d-pantolactone hydrolase production was sucrose and the best nitrogen source was corn steep liquor. The optimum temperature and the initial pH for enzyme formation was 26 °C and pH 7.0, respectively. When the fungus was cultured in a medium containing 30 g/L sucrose, 100 g/L corn steep liquor in the 10 L fermentor, 30.2 g dry cells/L and 410 U/L lactonohydrolase activity were obtained. For optical resolution, the optimum temperature and the optimum pH for the reaction with free cells was 50 °C and pH 7.0, respectively. The optimum substrate concentration was 200 g/L racemic pantolactone. The lactonohydrolase activity of immobilized cells remained at 80% of the original reaction after 20 batches of reuse.

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