Article ID Journal Published Year Pages File Type
36462 Process Biochemistry 2005 6 Pages PDF
Abstract

Ralstonia eutropha was cultivated in a continuous-stirred tank reactor to study the dynamic responses of PHBV production under pH-state. The effects of carbon sources (glucose and sodium propionate) and dilution rate on microbial growth and PHBV accumulation were studied. In feeding substrates containing less than 3 g/L of sodium propionate, PHB was the major product with only small amounts of HV accumulated. When the feeding substrates contained 5 g/L of sodium propionate, HV began to accumulate in the product, averaging a HB to HV molar ratio of 70:30. When the feed contained 7 g/L of sodium propionate, the average HB to HV molar ratio was approximately 60:40. Feeding substrate containing 15 g/L of sodium propionate had an average HB to HV molar ratio of 40:60. The continuous culture systems did not reach significant steady states when the concentration of sodium propionate exceeded 7 g/L.

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