Article ID Journal Published Year Pages File Type
36498 Process Biochemistry 2005 5 Pages PDF
Abstract

This paper describes a simple, fast and sensitive method for the determination of hydrogen peroxide in liquid samples. The system, consisting of a mini-reactor containing horseradish peroxidase (HRP) immobilised onto polyaniline coupled to a spectrophotometer, was able to determine hydrogen peroxide in the range from 55 to 2730 nmol L−1 using pyrogallol as the reduced substrate. The determinations were made using a flow injection system with a flow rate of 3.0 mL min−1 and optical detection at 420 nm with a glass flow cell having a 1 mm optical path length. The system has good response repeatability (R.S.D. 0.3–2.3%), while the mini-reactor can be stored and reused with up to 1500 injections without significant reduction of enzyme activity.

Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
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