Article ID Journal Published Year Pages File Type
36699 Process Biochemistry 2005 6 Pages PDF
Abstract

A novel surfactant- and oxidation-stable alkaline protease from Vibrio metschnikovii DL 33–51 was purified using acetone precipitation, Q-sepharose ion exchange and Sephacryl S-100 gel filtration chromatographic techniques. The enzyme had a relative molecular weight of 29.5 kDa by SDS polyacrylamide gel electrophoresis. The optimal temperature and pH were found to be 60 °C and 12.0, respectively. The protease was stable in a pH range of 9.0–11.0. The half-lives of protease recorded at 50 and 60 °C were 7.5 h and 30 min, respectively. The enzyme activity could be strongly activated by several commercially available laboratory surfactants (Triton X-100, Tween-80) and bleaches (H2O2, sodium hypochlorite), and showed a valuable stability towards them when their concentrations ranged up to 1.0%. Enzyme activity was increased up to 19 and 18% by Mn2+ and Ba2+, respectively.

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