Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
3897 | Biochemical Engineering Journal | 2010 | 7 Pages |
Abstract
A kinetic model able to adequately describe the accumulation of p-topaquinone in peroxidase-mediated oxidation of l-dopa was developed, and the rate constants for both enzymatic and non-enzymatic branch were estimated either experimentally or using a computing program for detailed kinetic simulation. It is demonstrated that the accumulation of p-topaquinone in significant amounts occurred in excess of hydrogen peroxide or during auto-oxidation of l-dopa, but changes in the ratio of initial concentrations of the reactants can reduce considerably the production of this intermediate.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Mihaela Puiu, Irina Babaligea, Cătalina Olmazu, Adina Răducan, Dumitru Oancea,