Article ID Journal Published Year Pages File Type
3982 Biochemical Engineering Journal 2010 9 Pages PDF
Abstract

With the aim to produce cellulases and to study the effect of mechanical agitation, a 35 L draft-tube airlift bioreactor equipped with a mechanical impeller was developed and validated to grow Trichoderma reesei RUT-C30 in a cellulose culture medium with lactose and lactobionic acid as fed batch. Cultures carried out without mechanical agitation resulted in higher volumetric enzyme productivity (200 U L−1 h−1), filter paper activity (17 U mL−1), carboxymethyl cellulase activity (11.8 U mL−1) and soluble proteins (3.2 mg mL−1) when compared to those with agitation. Stereo and polarized light microscopy analyses reveal that mechanical agitation resulted in shorter mycelial hyphae and larger numbers of tips.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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