Article ID Journal Published Year Pages File Type
3986 Biochemical Engineering Journal 2010 6 Pages PDF
Abstract

d-Amino acid oxidase from Rhodosporidium toruloides (RtDAO) and Fe3O4 magnetic nanoparticles were encapsulated simultaneously within biomimetic silica, as mediated by polyallylamine. The capacity for this enzyme reached 193 mg/g of biomimetic silica when 15 mg/ml RtDAO was used during encapsulation; the average encapsulation efficiency was approximately 74%. The Tm value (the temperature at which 50% of the initial activity was retained after 1 h of incubation) was increased from 44.3 °C of the free RtDAO to 57.7 °C, clearly indicating the thermal stability was improved by encapsulation. In the presence of 50 mM hydrogen peroxide, encapsulated RtDAO had a half-life of 148 min, an approximately 2-fold increase in resistance to hydrogen peroxide as compared to 78-min half-life of the free form. The encapsulation process is simple and can be completed within minutes; besides, the resultant enzymes can be recovered easily under magnetic field. Such preparation of encapsulated d-amino acid oxidase could be exploited for many potential applications.

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Physical Sciences and Engineering Chemical Engineering Bioengineering
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