Modelling and simulation of a transketolase mediated reaction: Sensitivity analysis of kinetic parameters

In this paper we have used a proposed mathematical model, describing the carbon–carbon bond formation reaction between β-hydroxypyruvate and glycolaldehyde to synthesise l-erythrulose, catalysed by the enzyme transketolase, for the analysis of the sensitivity of the process to its kinetic parameters. The model was validated with experimental data. As a conclusion, kinetic parameters with a possible positive impact on reaction performance were identified and assessed in relation to operating conditions. This resulted in the identification of suitable catalyst and process development targets.