Low-Abundance Biofilm Species Orchestrates Inflammatory Periodontal Disease through the Commensal Microbiota and Complement

SummaryPorphyromonas gingivalis is a low-abundance oral anaerobic bacterium implicated in periodontitis, a polymicrobial inflammatory disease, and the associated systemic conditions. However, the mechanism by which P. gingivalis contributes to inflammation and disease has remained elusive. Here we show that P. gingivalis, at very low colonization levels, triggers changes to the amount and composition of the oral commensal microbiota leading to inflammatory periodontal bone loss. The commensal microbiota and complement were both required for P. gingivalis-induced bone loss, as germ-free mice or conventionally raised C3a and C5a receptor-deficient mice did not develop bone loss after inoculation with P. gingivalis. These findings demonstrate that a single, low-abundance species can disrupt host-microbial homeostasis to cause inflammatory disease. The identification and targeting of similar low-abundance pathogens with community-wide impact may be important for treating inflammatory diseases of polymicrobial etiology.

Graphical AbstractFigure optionsDownload full-size imageDownload high-quality image (168 K)Download as PowerPoint slideHighlights► P. gingivalis induces changes to the amount and composition of the oral microbiota ► P. gingivalis causes periodontitis in conventional but not germ-free mice ► Complement is also required for P. gingivalis-induced periodontitis ► Defective leukocyte recruitment disrupts host-microbial homeostasis, causes bone loss