| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4370594 | Revista Argentina de Microbiología | 2013 | 4 Pages |
ResumenCon el objetivo de reducir el número de resultados falsos reactivos al determinar los anticuerpos contra HTLV-1/2, se evaluaron algoritmos alternativos. De 20 210 muestras estudiadas, el 0,37 % (74/20 210) fueron reactivas por ELISA Murex Abbott. De estas, 23 se confirmaron positivas por inmunofluorescencia indirecta y 51 fueron negativas; valor predictivo positivo (VPP) 31,08 %. Al combinar ELISA Murex con aglutinación de partículas de gelatina (AP) y con ELISA MP, se obtuvieron los siguientes resultados: 26/74 resultaron reactivas por Murex y AP, VPP 88,5 %, y 32/74 reactivas con Murex y ELISA MP, VPP 71,8 %. Del análisis de la curva ROC se determinó que para un valor de RP de 4,74 por Murex, la sensibilidad, la especificidad, el VPP y el VPN son 100 %, 98,04 %, 95,8 % y 100 %, respectivamente. Proponemos que las muestras reactivas por ELISA Murex con RP ≤ 4,74 sean retesteadas por duplicado por AP, y que las que resulten concordantemente no reactivas sean definidas como negativas para HTLV-1/2.
Alternative algorithms were evaluated in order to reduce the number of false reactive results for antibodies against HTLV-1/2. From 20,210 samples tested, 0.37% (74/20,210) was reactive by ELISA Murex. Of these, 23 were confirmed as positive by the indirect immunofluorescence assay whereas 51 were negative, being the positive predictive value (PPV) 31.08%. From a combination of the ELISA Murex assay with the particle agglutination assay (PA) and ELISA MP, the following results were obtained: 26/74 were reactive by ELISA Murex and PA, PPV 88.5% and 32/74 were reactive by ELISA Murex and ELISA MP, PPV 71.8%. The ROC curve analysis determined that for an RP 4.74, the values for sensitivity, specificity, PPV and NPV by ELISA Murex were 100%, 98.04%, 95.8% and 100%, respectively. We propose that reactive samples by ELISA Murex with an RP ≤ 4.74 should be retested in duplicate by PA, and the resulting concordantly nonreactive samples should be defined as negative for HTLV-1/2.
