| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 4437 | Biochemical Engineering Journal | 2008 | 6 Pages |
Abstract
Production and purification of starch digesting cyclodextrin glycosyl transferase (CGTase) from alkalophilic Bacillus firmus was investigated. Fermentation was carried out in 14 l bioreactor at 28 °C using a medium containing dextrin, yeast extract, peptone, (NH4)H2PO4 and MgSO4·7H2O. The extracellular enzyme was concentrated by tangential flow ultrafiltration. The concentrated enzyme was chromatographed using DEAE-sepharose and phenyl sepharose. DEAE-sepharose could be used to purify CGTase in a single step with 23.1 fold purification and 80.6% recovery. The enzyme obtained had homogeneity and the molecular weight was 76 kDa confirmed by SDS-PAGE.
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Authors
Laxman S. Savergave, Santosh S. Dhule, Vitthal V. Jogdand, Sanjay N. Nene, Ramchandra V. Gadre,