Quantitative coherent anti-Stokes Raman scattering microspectroscopy using a nanosecond supercontinuum light source

We describe and characterize a multiplex CARS microspectroscopic system that uses a nanosecond supercontinuum generated from a photonic crystal fiber and a sub-nanosecond pulse laser. This system has a high spectral resolution (<0.1 cm−1) and an ultrabroadband spectral coverage (>2500 cm−1). The estimated spatial resolutions are 0.45 μm (lateral) and 4.5 μm (axial), respectively. This system enables us to obtain CARS spectra and corresponding images in the fingerprint region as well in the CH stretch region. Using this system, we have successfully obtained label-free and multi-mode vibrational images of a yeast cell.

► Multiplex CARS microspectroscopy using a nanosecond supercontinuum light source. ► Characteristics of the CARS microspectroscopic system. ► Label-free and multi-modal imaging of a living cell.