Elimination of 4-chlorophenol by soybean peroxidase and hydrogen peroxide: Kinetic model and intrinsic parameters

A kinetic model for the initial rate reaction, based on the Ping-Pong Bi-Bi mechanism, has been assumed for the removal of 4-chlorophenol with soybean peroxidase and hydrogen peroxide. Several sets of experimental data, obtained in a batch reactor at different concentrations of hydrogen peroxide and 4-chorophenol, were used to estimate the kinetic parameters. A new method for determining the intrinsic parameters of the reaction in processes involving a high reaction rate was developed. The method consists of determining several sets of apparent parameters at different times and then extrapolating these to zero time.