Hot pressurized water extraction of syringin from Acanthopanax senticosus and in vitro activation on rat-blood macrophages

This work studied the effect of hot pressurized water extraction of Acanthopanax senticosus (AS) roots on the activity, phagocytosis and nitric oxide (NO) molecules releasing of rat peripheral blood macrophages. Experimental results indicated that a kind of phenyl-propanoid glycosides, i.e., syringin plays a role in stimulating rat-blood macrophages to perform phagocytosis. Suitable concentrations of the syringin standard in the aqueous solution, ranging from 4 to 16 mg/L, notably sustain the activity of macrophages, but speed up the releasing of NO molecules from macrophages after incubation from 24 to 72 h. A two-factor central composite experimental designed hot pressurized water extractions of A. senticosus indicated that the enhancement of phagocytosis after the 24, 48 and 72 h incubations may be induced by a co-solute effect of syringin and other ingredients in the extracts.