Short communicationFed-batch fermentation with intermittent gas stripping using immobilized Clostridium acetobutylicum for biobutanol production from corn stover bagasse hydrolysate

•Corn stover was used as feedstock and immobilized carrier.•Fermentation was integrated with gas stripping for cellulosic ABE production.•115-220 g/L of ABE in the condensate of intermittent gas stripping.•The phenols showed inhibition to the integrated fermentation process.

Corn stover bagasse hydrolysate was used as the substrate for fed-batch acetone-butanol-ethanol (ABE) fermentation and in situ product removal by intermittent gas stripping. The mutant strain Clostridium acetobutylicum ABE-P 1201 was inoculated for ABE production. As results, a final cumulative butanol concentration of 18.6 g/L (total ABE: 28.3 g/L) was produced from 97.6 g/L of total fermentable sugars (75.3 g/L of glucose and 22.3 g/L of xylose), with an ABE yield of 83.2 g/kg of CSB. The gas stripping condensate obtained in the integrated process contained 77-136 g/L of butanol and 115-220 g/L of total ABE.