Synthesis of silver nanoparticles (Ag NPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum

•Silver nanoparticles (Ag-NPs) synthesized by natural medicinal Piper nigrum extract•Bioactivity test for in-vitro cytotoxicity efficacy against MCF-7 & HEP-2 cells•Ag-NPs formed within 20 min and confirmed by UV-Visible spectroscopy•UV-Vis showed surface Plasmon resonance (SPR) peak ~ 441 nm observed•Ag-NPs surface HRTEM images shows spherical shape with particle-size 5 to 20 nm.•MTT assays for cytotoxicity carried-out different concentration of the plant extract.

In the present report, silver nanoparticles were synthesized using Piper nigrum extract for in vitro cytotoxicity efficacy against MCF-7 and HEP-2 cells. The silver nanoparticles (AgNPs) were formed within 20 min and after preliminarily confirmation by UV-Visible spectroscopy (strong peak observed at ~ 441 nm), they were characterized by using FT-IR and HR-TEM. The TEM images show spherical shape of biosynthesized AgNPs with particle size in the range 5-40 nm while as compositional analysis were observed by EDAX. MTT assays were carried out for cytotoxicity of various concentrations of biosynthesized silver nanoparticles and Piper nigrum extract ranging from 10 to 100 μg. The biosynthesized silver nanoparticles showed a significant anticancer activity against both MCF-7 and Hep-2 cells compared to Piper nigrum extract which was dose dependent. Our study thus revealed an excellent application of greenly synthesized silver nanoparticles using Piper nigrum. The study further suggested the potential therapeutic use of these nanoparticles in cancer study.

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