Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
4755099 | Process Biochemistry | 2017 | 15 Pages |
Abstract
During the past two decades, there has been an explosion of new knowledge and techniques in the field of recombinant protein expression. However, over-expression of “difficult to express proteins” with therapeutic importance continues to be a challenging task for successful commercialization of these proteins. With the emergence of the bio-similar market, enhancing the efficiencies of the production process has become a critical factor in the commercial viability of novel products. Despite the availability of numerous technological advancements, recombinant protein expression in Escherichia coli remains difficult. Therefore, addressing upstream bottlenecks in combination with genetically modified expression hosts could be a viable strategy to enhance production. Problems like poor expression, plasmid instability, protein aggregation, protein degradation, and metabolic stress associated with recombinant protein production need special consideration during bioprocess development at bioreactor level. However, a comprehensive universal strategy for attaining efficient expression in E. coli seems unrealistic and must be resolved empirically. In this review, we have discussed some common problems and their apparent solutions for plasmids based recombinant gene expression in E. coli.
Keywords
T7 RNAPpSKE. coliShine-DalgarnoTrxPhoAPNPaseDsbCNusASRPPfg27GSTIPTGRBSSUMORFSCSPORIT7 RNA polymeraseAlkaline phosphataseEscherichia coliInductionMetabolic burdenExpression vectorTATTwin-arginine translocationTIRthioredoxinsignal recognition particleRibosomal binding siteRelease factorstrigger factorPolynucleotide phosphorylaseORTorigin of replicationTranslation initiation regionStress responsespelBcold shock proteinphage shock proteinRecombinant proteinPromoterPlasmidsmall ubiquitin-like modifierglutathione S-transferase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Tapan Kumar Singha, Pooja Gulati, Aparajita Mohanty, Yogender Pal Khasa, Rajeev Kumar Kapoor, Sanjay Kumar,