Article ID Journal Published Year Pages File Type
4760280 Forensic Science International 2017 5 Pages PDF
Abstract

•Fingermarks were deposited on glass slides.•Some fingermarks were treated with fpNatural 1™ powder and some were left untreated.•No difference between DNA yield in treated or untreated fingermarks.•No difference between qualitative allele recovery in treated or untreated fingermarks.•fpNatural 1™ allows the subsequent generation of DNA profiles from fingermarks.

The effect of the infrared fluorescent fingermark visualisation powder, fpNatural 1™, on the recovery of both the quantity and quality of touch DNA from fingerprints deposited on glass slides, was investigated using qPCR and STR typing. Four donors each deposited replicate marks, which were either left untreated (n = 5) or treated by dusting with fpNatural 1™ (n = 5). Each sample was swabbed using the double swab technique, before being extracted using the EZNA Forensic DNA kit and then DNA quantitated before being subjected to DNA profile analysis. Results showed that there was no significant effect of fpNatural 1™ on either the quantity or quality of recovered DNA. This suggests that fpNatural 1™ may prove a good choice of powder for regular use at crime scenes or in the laboratory. The fpNatural 1™ properties of low density, water immiscibility and low DNA affinity may account for these positive outcomes.

Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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